The Titanium Sapphire laser is used for several applications due to its generation of short pulse times and high peak power. The broad tuning range, enhanced efficiency and high thermal conductivity are also considered when deciding its operations. It has applications in precision materials processing, beaming power to satellites, generating soft x-rays for x-ray lithography, and medical fields.

The laser can be used to clean buildings and sculptures by selectively removing particular coatings without harm to anything else. It has even been used to measure water vapour, pressure and temperature. Medical applications involve enriching stable medical isotopes (such as Carbon 13 and Oxygen 18), treating different areas of pigments associated with dermatology, and aiding the correction of kidney problems when guided by optical fibers (a medical procedure known as Lithotripsy).